Box Behnken Design Software Free Download
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Formulation optimization process was carried out using a Box-Behnken design, as it requires few runs with three or four variables. Here three variables at three levels were studied using total 17 runs.[16] Layout of the Box-Behnken design is represented in Table 2. Effect of three factors X1 (HPMC K-15), X2 (Maltodextrin) and X3 (PVA) on mechanical properties of film and percentage drug release in 30 s (Q30) in acidic condition were studied by Box-Behnken design. A set of points lying at the midpoints of each edge of the multidimensional design cube as well as replicated center points were utilized to construct mathematical models and response surfaces using Design Expert® software (Version 7.0.0, Stat-Ease Inc., Minneapolis, MN, USA). Derived models were validated using three checkpoint batches CHI^-CHI^.
Chromium which is a heavy metal component of SEO has been designated as a priority pollutant by US EPA[8] and found to be toxic, carcinogenic, and teratogenic when present at high concentration[9]. Chromium (Cr) does not occur naturally in elemental form, only in compounds. Chromium exists in nine valence states ranging from 2 to +6; nevertheless, trivalent chromium (Cr (III)) and hexavalent chromium (Cr (VI)) are of major environmental significance because of their stability in the natural environment[10]. Hexavalent chromium (chromate) compounds are highly water soluble and, therefore, can overcome the cellular permeability barrier, entering via sulfate transport pathways since it bears structural similarity with SO4. It has a high tendency to bind with oxygen, and unless it is rapidly reduced, it can oxidatively damage the DNA via the production of free radicals[9]. It has been reported that hexavalent chromium causes lung cancer[11], chromate ulcer, perforation of nasal septum, and kidney damage in humans, and it is also toxic to other organisms as well. Trivalent chromium is an essential micronutrient for many microorganisms, relatively insoluble in water, and 100 times less toxic than the hexavalent form.
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